Enzyme Assay
Enzymes are very efficient catalysts for biochemical reactions. They speed up reactions by providing an alternative reaction pathway of lower activation energy.Enzymes have an active site. This is part of the molecule that has just the right shape and functional groups to bind to one of the reacting molecules. The reacting molecule that binds to the enzyme is called the substrate.
MATERIALS
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APPARATUS
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PROCEDURE
The Effect of Temperature
The following table below is uses as guide in an experiment the effect of temperature on enzyme.
The Effect of Temperature
The following table below is uses as guide in an experiment the effect of temperature on enzyme.
1. We examine 4 different temperature which is 20,28,30 and 40 celcius.
2.We determine which best temperature for enzyme reaction.
2.We determine which best temperature for enzyme reaction.
CONCLUSION
In the nutshell, if the temperature rises, molecular motion and hence collisions between enzyme and substrate speed up. But as enzymes are proteins, there is an upper limit beyond which the enzyme becomes denatured and ineffective.